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Stat3基因特异性短发夹RNA干扰质粒和GRIM-19对PC-3M肿瘤细胞生长的影响

Effects of plasmid-based Stat3-specific short hairpin RNA and GRIM-19 on PC-3M tumor cell growth
Zhang L., Gao L., Li Y., Lin G., Shao Y., Ji K., Yu H., Hu J., Kalvakolanu D.V., Kopecko D.J., Zhao X., Xu D.-Q.   |   2009/5/29 18:40:37 
Clinical Cancer Research, 2008   |   Volume 14, Issue 2  |   打印| 推荐给好友
上一篇: 雷帕霉素单用及联合索拉非尼在人肝细胞肝癌原位模型的疗效
下一篇: 第3章：FAK基因表达——调节和治疗的潜力
Purpose: Persistent activation of signal transducers and activators of transcription 3 (Stat3) and its overexpression contribute to the progression and metastasis of several different tumor types. For this reason, Stat3 is a reasonable target for RNA interference - mediated growth inhibition. Blockade of Stat3 using specific short hairpin RNAs (shRNA) can significantly reduce prostate tumor growth in mice. However, RNA interference does not fully ablate target gene expression in vivo, owing to the idiosyncrasies associated with shRNAs and their targets. To enhance the therapeutic efficacy of Stat3-specific shRNA, we applied a combination treatment involving gene associated with retinoid-IFN - induced mortality 19 (GRIM-19), another inhibitor of STAT3, along with shRNA. Experimental Design: The coding sequences for GRIM-19, a cellular STAT3-specific inhibitor, and Stat3-specific shRNAs were used to create a dual expression plasmid vector and used for prostate cancer therapy in vitro and in mouse xenograft models in vivo. Results: The coexpressed Stat3-specific shRNA and GRIM-19 synergistically and more effectively suppressed prostate tumor growth and metastases when compared with treatment with either single agent alone. Conclusion: The simultaneous use of two specific, but mechanistically different, inhibitors of STAT3 activity exerts enhanced antitumor effects. © 2008 American Association for Cancer Research.
Correspondence Address: Zhao, X.; Department of Pathophysiology, School of Basic Medicine, Jilin University, Xinmin Street, Changchun, 130021, China; email: pro_2@jlu.edu.cn 
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慢性心衰诊治：规范中求突破
黄峻
2012-2-1
南京医科大学第一附属医院
房颤治疗：手段渐趋丰富 新型治疗药物不断涌现 非药物治疗备受关注
马长生
2012-2-1
首都医科大学附属北京安贞医院
注重老年人群特征 优化管理

刘梅林
2012-2-1
北京大学第一医院老年内科

 
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