Background: Kaiso is a recently identified transcription factor that binds to p120-catenin (p120ctn), an Armadillo catenin and cell adhesion cofactor. However, clinical studies of human solid tumors have not been reported to investigate relationship between these proteins. Methods: Expression and localization of Kaiso and p120ctn were examined in 196 lung cancer specimens (including 55 cases of paired lymph node metastases and 80 cases with complete follow-up records) by immunohistochemistry. Three lung cancer cell lines, BE1, SPC, and A549 were used to establish p120ctn stably ablated or overexpressed cell lines. Co-immunoprecipitation was used to confirm p120ctn bind Kaiso in lung cancer tissue and cell lines. Localization and expression levels of Kaiso were detected via immunofluorescence, cytoplasmic vs. nuclear fractionation Western blot analysis and reverse transcription-polymerase chain reaction. Results: Cytoplasmic Kaiso expression was evident in 115 (58.7%), and abnormal p120ctn expression was noted in 168 (85.7%). Cytoplasmic Kaiso and abnormal p120ctn expressions were associated with higher degree of malignancy (high-stage and lymph node metastases, all P < 0.05). Abnormal p120ctn and cytoplasmic Kaiso expressions were higher in matched autologous nodal metastases than in primary growths. The lung cancer-related 5-year survival rate was significantly lower in patients who were cytoplasmic Kaiso-positive (22.9%; P = 0.029) or abnormal p120ctn expression (20.6%; P = 0.001). Multivariate analysis showed abnormal p120ctn expression was an independent factor defining the clinicopathological characters of patients. Cytoplasmic Kaiso expression was correlated with cytoplasmic p120ctn, they formed Kaiso-p120ctn complex in lung cancer tissues and cell lines. In addition, p120ctn ablation and overexpression altered Kaiso subcellular localization and protein level. Although both isoforms can regulate subcellular localization and protein levels of Kaiso, we found that only p120ctn isoform 3, but not isoform 1, directly interacts with Kaiso. Conclusion: p120ctn and Kaiso might co-participate in the progression and lymph node metastasis of lung cancer. p120ctn regulates expression and localization of Kaiso in lung cancer cells. © 2009 Elsevier Ireland Ltd. All rights reserved.
刘梅林 2012-2-1 北京大学第一医院老年内科
友情链接:中文版柳叶刀 | MD CONSULT | Journals CONSULT | Procedures CONSULT | eClips CONSULT | Imaging CONSULT |
爱爱医 | 好大夫 | 医师网 | 丁香园 | 论文吧 | 世界医学书库 | 好医生论坛 | 医心网 | 前沿医学网 | 白天使 | 医脉通 |
360网址导航 | 中华外科杂志 | 中国妇产科网 | 骨科在线 | 新青年麻醉论坛 | 中华泌尿外科学会网 | 公司简介 | 用户协议 | 条件与条款 | 隐私权政策 | 联系我们 互联网药品信息服务资格证书 | 卫生局审核意见通知书 | 药监局行政许可决定书 电信与信息服务业务经营许可证 | 京ICP证070259号 | 京ICP备09068478号
Copyright © 2009 Elsevier. All Rights Reserved. 爱思唯尔版权所有