This study aimed to investigate the location and function of tau isoform of 14-3-3 proteins in human trophoblast. 14-3-3 tau was localized in human cytotrophoblast cells, but not in syncytiotrophoblast cells in both first trimester and term placenta by immunochemistry stain. Forskolin-induced cell fusion (BeWo cells) confirmed that 14-3-3 tau was decreased during trophoblast differentiation. Forskolin-induced differentiation was stimulated by small-interfering (si) RNA induced down-regulation of 14-3-3 tau, contrarily, it was suppressed by plasmid induced upregulation of 14-3-3 tau in BeWo cells. When BeWo cells were treated with 14-3-3 tau siRNA, an increase in protein concentration of cell cycle inhibitor p27kip1 and a decrease in protein concentration of proliferating cell nuclear antigen, as well as activation of the extracellular signal-regulated kinase (ERK) pathway, were also noticed. These findings suggest that 14-3-3 tau might be mediated trophoblast differentiation through cell cycle regulation. © 2009 Elsevier Ltd. All rights reserved.
摘自:《脑电图精粹》(主译:元小冬 许亚茹),北京大学医学出版社出版
男孩,8岁,出现双侧上肢和躯干抽搐发作,没有明显的意识改变。患者具有病毒性脑膜脑炎后的严重抑制性脑病,并出现失明和四肢瘫。患者没有应用中枢神经系统兴奋剂。
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Copyright © 2009 Elsevier. All Rights Reserved.爱思唯尔版权所有